This is an assessment of the distribution and levels of contaminants of emerging concerns (CECs) in the surface water of Imo River, Nigeria using the non-target approach. Three (3) locations -  Ekenobizi (Imo State), Owerrinta (Abia State) and Oyigbo (Rivers State) along the  river were sampled based on high human density with much anthropogenic activities. Surface water samples were collected from upstream, midstream and downstream of each of the locations using the grab sampling method and taken to the laboratory for analysis within three hours after sampling. A non- targeted screening was done using gas chromatography – mass spectrometry (GC-MS) after preparation of the samples. The results showed contamination of the river with eighty – five (85) unique CECs distributed as follows; 21 CECs at Ekenobizi axis, 27 CECs at Owerrinta axis and 40 CECs at Oyigbo axis. Some of the CECs were location specific while some were detected across the three sampling points and locations. Among the detected compounds were siloxanes, fatty acids, amines, alkanes/alkenes, fluorinated organic compounds, Nitrogenous based compounds and other organic chemicals. These chemicals are traceable to pharmaceutical and personal care products, agro-chemicals, and other industrial chemicals and they exhibit varying degrees of ecological risks.